ULTRACENTRIFUGAL STUDIES OF LIPOPROTEINS OF HUMAN SERUM

Ultracentrifugal studies of lipoproteins of human serum.

In spite of several extensive ultracentrifugal studies of human sera by Mutaenbecher, McFarlane, and Pedersen, the interpretation of the patterns observed has remained in doubt. Specifically, the greatest difficulties encountered by previous workers have arisen in their efforts to study undiluted human sera. McFarlane (1) referred to marked distortions in the pattern observed with undiluted hum...

Ultracentrifugal studies of high density serum lipoproteins in clinically healthy adults.

INDGREN and co-workers (I) have described L the properties and methodology for the ultracentrifugal study of the entire spectrum of lipoproteins present in human serum. The three most dense lipoproteins described by Lindgren et al. represent the subject of present considerations. These lipoproteins have been identified by their estimated hydrated densities as a lipoprotein of density 1.05 gm/ml...

Studies on Human Serum High Density Lipoproteins

Human serum apolipoprotein A-I (ape-A-I), the major protein component of the human serum high density lipoproteins, was studied in aqueous solutions of differing ionic strengths and pH by the techniques of sedimentation equilibrium ultracentrifugation and frontal analysis gel chromatography. The ultracentrifugal studies indicate that apo-A-I is a self-associating system that is dependent upon p...

Ultracentrifugal studies of major non-lipide electrophoretic components of normal human serum.

In the ultracentrifuge, normal human serum shows, in addition to the lipoproteins, three distinct components with sedimentation rates of approximately 4, 7, and 19 Svedberg units (1, 2). Other materials known to be present are obscured by the high concentrations of these major substances and the high diffusion spreading of the boundaries. For example, Edsall (3) has reported a number of additio...

Electrophoretic and ultracentrifugal analysis of serum lipoproteins of normal, nephrotic and hypertensive persons.